Intermediate

Part:BBa_K2259027:Design

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-02)


SynORI constitutive plasmid copy number device intermediate (0.86 Anderson)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutations for group selection had to be carefully tailored in order not to change secondary structure of RNA I.


Source

This part is a modified version of wildtype ColE1 RNA I taken from addgene.

References